Immunological characterization of a diabetogenic protein from bovine adenohypophysis.

l. A diabetogenic protein isolated from bovine adenohypophysis which had previously been demonstrated to cause fasting hyperglycemia and decreased glucose tolerance in dogs and man is highly antigenic when injected into rabbits. 2. Immunoelectrophoresis and gel double diffusion studies did not provide evidence of multiple antibodies or antigens in the bovine diabetogenic protein: anti-bovine diabetogenic system. 3. Results of agar diffusion studies in which anti-bovine diabetogenic protein serum did not visibly react with homologous bovine serum proteins, serum albumin, ACTH or prolactin lend supporting evidence for the homogeneity of the reaction between bovine diabetogenic protein and its antiserum. 4. A comparative immunological study of diabetogenic proteins from various species was performed using agar double diffusion studies